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KMID : 0359319930330020345
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Korean Journal of Veterinary Research
1993 Volume.33 No. 2 p.345 ~ p.350
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Effects of glycerol and seminal plasma in characteristics of preserved canine spermatozoa
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Abstract
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Multiple ejaculates were collected from four male mongrel dogs. The second fraction and the small volume of third fraction from the ejaculates were divided and treated as follows : control; addition of the egg-yolk Tris extender to the semen at 37℃. group I ; Removal of seminal plasma, group II ; addition of the glycerolated extender at 4℃, group III Removal of seminal plasma and addition of glycerolated extender at 4℃. The semen cooled to 4℃ was equlibrated for 2hrs and preserved in refrigerator at 4℃. The preserved semen was evaluated for kinetics, morphology, motility and thermoresistance daily for 3 days.
1. The kinectics after preserved days 2 and 3 of group I was significantly higher than that of control(p<0.05).
2. There were no significant difference in abnormal morphology of each group between the periods of storage.
3. The motility after preserved day 1 and days 3 of group I was significantly higher than that of others(p<0.05), and the molity after preserved days 2 of group I and III was signficantly higher than that of others(p<0.05).
4. When the molity of preserved semen was measured during incubation at 37℃, the motility of four groups was declined at similar rates. There was no effect of removal of seminal plasma and glycerol addition on thermoresistance.
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